Daniel Kopinke

Daniel Kopinke, Ph.D.

AST PROF

Department: MD-PHARMACOLOGY / THERAPEUTICS
Business Phone: (352) 294-5355
Business Email: dkopinke@ufl.edu

Research Profile

Visit website for more detail @ www.kopinkelab.com

In many tissues, wound healing and regeneration depends on stem cells to replace the lost or damaged cells. In injured skeletal muscle, a dedicated muscle stem cell population gives rise to new muscle myofibers after an acute injury. In chronic diseases, however, muscle regeneration fails and healthy muscle is gradually replaced with fibrotic scar and fat tissue, a process called fatty fibrosis. This fatty fibrosis of muscle is a prominent feature of chronic muscle diseases such as Duchenne muscular dystrophy (DMD), sarcopenia (age-related loss of skeletal muscle and strength), obesity and diabetes. There are no cures for DMD and no specific therapies for either DMD or sarcopenia.

Coordinating cell-cell interactions is critical for regenerating complex tissues after injury or disease. Primary cilia are small, immotile, microtubule-based cell projections and have evolved to receive and interpret extracellular cues. Cilia play a crucial role in intercellular communication during development and defects in cilia lead to embryonic lethality in both mice and humans. While cilia are present on the majority of cells in our body, there’s little known about how they function or participate in the repair of adult tissues.

It was recently discovered that cilia coordinate muscle repair by controlling the communication between the muscle stem cell population and its support cells. The Kopinke Lab is now building on this work by investigating how ciliary signaling coordinates cellular communication between stem cells and their niche, to understand how cilia-based communication goes awry in disease and to identify novel pharmacological tools to combat cilia-associated diseases such as fatty fibrosis.

Areas of Interest
  • Regenerative and Precision Therapeutics
  • Stem Cell biology
  • Tissue engineering related to wound healing

Publications

2021
Automatic and unbiased segmentation and quantification of myofibers in skeletal muscle.
Scientific reports. 11(1) [DOI] 10.1038/s41598-021-91191-6. [PMID] 34083673.
2021
Developmental and regenerative paradigms of cilia regulated hedgehog signaling.
Seminars in cell & developmental biology. 110:89-103 [DOI] 10.1016/j.semcdb.2020.05.029. [PMID] 32540122.
2019
Disulfide bond-disrupting agents activate the tumor necrosis family-related apoptosis-inducing ligand/death receptor 5 pathway.
Cell death discovery. 5 [DOI] 10.1038/s41420-019-0228-9. [PMID] 31839995.
2019
Omega-3 Fatty Acids Activate Ciliary FFAR4 to Control Adipogenesis.
Cell. 179(6):1289-1305.e21 [DOI] 10.1016/j.cell.2019.11.005. [PMID] 31761534.
2017
Ciliary Hedgehog Signaling Restricts Injury-Induced Adipogenesis
.
2017
Ciliary Hedgehog Signaling Restricts Injury-Induced Adipogenesis.
Cell. 170(2):340-351.e12 [DOI] 10.1016/j.cell.2017.06.035. [PMID] 28709001.
2013
Keratin 79 identifies a novel population of migratory epithelial cells that initiates hair canal morphogenesis and regeneration.
Development (Cambridge, England). 140(24):4870-80 [DOI] 10.1242/dev.101725. [PMID] 24198274.
2012
Ongoing Notch signaling maintains phenotypic fidelity in the adult exocrine pancreas.
Developmental biology. 362(1):57-64 [DOI] 10.1016/j.ydbio.2011.11.010. [PMID] 22146645.
2012
Wnt signaling regulates postembryonic hypothalamic progenitor differentiation.
Developmental cell. 23(3):624-36 [DOI] 10.1016/j.devcel.2012.07.012. [PMID] 22975330.
2011
Lineage tracing reveals the dynamic contribution of Hes1+ cells to the developing and adult pancreas.
Development (Cambridge, England). 138(3):431-41 [DOI] 10.1242/dev.053843. [PMID] 21205788.
2010
Exocrine-to-endocrine differentiation is detectable only prior to birth in the uninjured mouse pancreas.
BMC developmental biology. 10 [DOI] 10.1186/1471-213X-10-38. [PMID] 20377894.
2009
Lhx2 links the intrinsic and extrinsic factors that control optic cup formation.
Development (Cambridge, England). 136(23):3895-906 [DOI] 10.1242/dev.041202. [PMID] 19906857.
2008
Pancreatic stem cells
StemBook. [PMID] 20614618.
View on: PubMed
2006
Retinoic acid is required for endodermal pouch morphogenesis and not for pharyngeal endoderm specification.
Developmental dynamics : an official publication of the American Association of Anatomists. 235(10):2695-709 [PMID] 16871626.
View on: PubMed

Grants

Jul 2015 ACTIVE
Research Pharmacology V
Role: Project Manager
Funding: UF FOU
Jul 2015 – Jun 2021
Research Pharmacology VII
Role: Project Manager
Funding: UF FOU

Teaching Profile

Courses Taught
2019-2021
GMS6531 Medical Pharmacology and Therapeutics III: Endocrine, Musculoskeletal and Reproductive Systems
2020-2021
PAS5026 Pharmacotherapeu 2
2020-2021
DEN6262 Prin of Pharmacology
2019,2021
GMS6013 Developmental Genetics
2021
ZOO4926 Special Topics in Zoology

Contact Details

Phones:
Business:
(352) 294-5355
Emails:
Business:
dkopinke@ufl.edu