Lal C Garg, Ph.D.
Professor Emeritus
Department:
MD-PHARMACOLOGY / THERAPEUTICS
Business Phone:
(352) 378-4623
Business Email:
lcgarg@ufl.edu
Teaching Profile
Courses Taught
2006-2007,2012
DEN6262 Prin of Pharmacology
2007
BMS6404 Pharmacology
Research Profile
Garg has researched elucidation and regulation of the biochemical mechanisms of electrolyte transport in various organs. In the 1970s he discovered a new isoenzyme of carbonic anhydrase, an enzyme involved in the acid-base balance of the body. In the 1980s and 1990s, while studying kidney function, he developed a system for measuring a sodium-transporting enzyme in the kidney’s nephron segments and demonstrated that mineral ocorticoids, steroids found in the adrenal cortex, regulate this enzyme in a few specific areas.
He also discovered an acid-transport system in the kidney that was previously thought to be present only in the stomach, and demonstrated that cholinergic drugs, used to treat conditions including glaucoma and severe muscle weakness, affect electrolyte transport in the kidney.
Garg said he enjoyed working with students and got a lot of satisfaction out of teaching. After retirement, he plans to travel and to continue to contribute to education efforts in the department of pharmacology and therapeutics.
(SOURCE: UFHealth, https://ufhealth.org/news/2003/lal-c-garg-phd-professor-pharmacology-and-therapeutics-retires)
Publications
2013
Recombinant expression of in silico identified Bcell epitope of epsilon toxin of Clostridium perfringens in translational fusion with a carrier protein.
Bioinformation.
9(12):617-21
[DOI] 10.6026/97320630009617.
[PMID] 23904738.
2012
Heterologous expression and biochemical characterization of recombinant alpha phosphoglucomutase from Mycobacterium tuberculosis H37Rv.
Protein expression and purification.
85(1):117-24
[DOI] 10.1016/j.pep.2012.07.004.
[PMID] 22809717.
2011
The Rv1651c-encoded PE-PGRS30 protein expressed in Mycobacterium smegmatis exhibits polar localization and modulates its growth profile.
FEMS microbiology letters.
322(2):194-9
[DOI] 10.1111/j.1574-6968.2011.02354.x.
[PMID] 21732963.
2010
Development of a recombinant epsilon toxoid vaccine against enterotoxemia and its use as a combination vaccine with live attenuated sheep pox virus against enterotoxemia and sheep pox.
Clinical and vaccine immunology : CVI.
17(6):1013-6
[DOI] 10.1128/CVI.00013-10.
[PMID] 20427629.
2010
Functional and structural characterization of soluble recombinant epsilon toxin of Clostridium perfringens D, causative agent of enterotoxaemia.
Applied microbiology and biotechnology.
88(4):877-84
[DOI] 10.1007/s00253-010-2785-y.
[PMID] 20676627.
2010
Identification and modeling of a drug target for Clostridium perfringens SM101.
Bioinformation.
4(7):278-89
[PMID] 20978600.
2010
Sequence analysis of 16S rRNA gene inRhinosporidium seeberi shows similarity to plant chloroplast DNA.
Bioinformation.
5(3):89-96
[PMID] 21364787.
2010
Structural studies of phosphoglucose isomerase from Mycobacterium tuberculosis H37Rv.
Acta crystallographica. Section F, Structural biology and crystallization communications.
66(Pt 5):490-7
[DOI] 10.1107/S1744309110011656.
[PMID] 20445242.
2008
Secretory and GM1 receptor binding role of N-terminal region of LTB in Vibrio cholerae.
Biochemical and biophysical research communications.
376(4):770-4
[DOI] 10.1016/j.bbrc.2008.09.066.
[PMID] 18814842.
2007
Crystallization and preliminary X-ray characterization of phosphoglucose isomerase from Mycobacterium tuberculosis H37Rv.
Acta crystallographica. Section F, Structural biology and crystallization communications.
63(Pt 4):353-5
[PMID] 17401215.
2007
Deletion mutations in N-terminal alpha1 helix render heat labile enterotoxin B subunit susceptible to degradation.
Proceedings of the National Academy of Sciences of the United States of America.
104(41):16056-61
[PMID] 17911243.
2007
Functional phosphoglucose isomerase from Mycobacterium tuberculosis H37Rv: rapid purification with high yield and purity.
Protein expression and purification.
52(2):373-8
[PMID] 17126561.
2006
Biochemical and functional characterization of triosephosphate isomerase from Mycobacterium tuberculosis H37Rv.
FEMS microbiology letters.
263(2):229-35
[PMID] 16978361.
2006
Chromosomal localization, copy number assessment, and transcriptional status of BamHI repeat fractions in water buffalo Bubalus bubalis.
DNA and cell biology.
25(4):206-14
[PMID] 16629593.
2006
Detection of 16S rRNA gene in round bodies isolated from polyps of rhinosporidiosis.
Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases.
6(4):331-6
[PMID] 16418014.
2006
Organizational and expressional uniqueness of a testis-specific mRNA transcript of protooncogene c-kit receptor in water buffalo Bubalus bubalis.
DNA and cell biology.
25(9):501-13
[PMID] 16989573.
2006
Transcriptional status of known and novel genes tagged with consensus of 33.15 repeat loci employing minisatellite-associated sequence amplification (MASA) and real-time PCR in water buffalo, Bubalus bubalis.
DNA and cell biology.
25(1):31-48
[PMID] 16405399.
2005
Biochemical characterization of recombinant phosphoglucose isomerase of Mycobacterium tuberculosis.
Biochemical and biophysical research communications.
337(2):626-32
[PMID] 16212940.
2005
Isolation, solubilization, refolding, and chromatographic purification of human growth hormone from inclusion bodies of Escherichia coli cells: a case study.
Methods in molecular biology (Clifton, N.J.).
308:163-76
[PMID] 16082034.
2001
Preventing and treating infections related to dental implant placement. Interview.
Dental implantology update.
12(10):73-7
[PMID] 11764601.
2000
Optimization of inclusion body solubilization and renaturation of recombinant human growth hormone from Escherichia coli.
Protein expression and purification.
18(2):182-92
[PMID] 10686149.
2000
Rapid communication: nucleotide sequence of the river buffalo beta-casein cDNA.
Journal of animal science.
78(5)
[PMID] 10834597.
2000
Rapid communication: nucleotide sequence of the river buffalo kappa-casein cDNA.
Journal of animal science.
78(5)
[PMID] 10834596.
1999
cDNA cloning and sequence analysis of bubaline growth hormone.
DNA sequence : the journal of DNA sequencing and mapping.
10(2):101-3
[PMID] 10376211.
1999
Effect of the codon following the ATG start site on the expression of ovine growth hormone in Escherichia coli.
Protein expression and purification.
17(2):215-23
[PMID] 10545269.
1999
Molecular cloning and sequence analysis of bubaline lactoferrin promoter.
DNA sequence : the journal of DNA sequencing and mapping.
10(2):97-9
[PMID] 10376210.
1999
Molecular cloning and sequence analysis of the cDNA encoding beta-lactoglobulin in Bubalus bubalis.
DNA sequence : the journal of DNA sequencing and mapping.
10(2):105-8
[PMID] 10376212.
1999
N-terminus of mature heat-labile enterotoxin chain B is critical for its extracellular secretion in Vibrio cholerae.
FEBS letters.
463(3):336-40
[PMID] 10606749.
1998
Expression and stable germline transmission of neomycin-resistance gene in transgenic mice.
Indian journal of experimental biology.
36(12):1209-15
[PMID] 10093502.
1998
Isolation and separation of HMG proteins and histones H1 and H5 and core histones by column chromatography on phosphocellulose.
Protein expression and purification.
14(2):155-9
[PMID] 9790876.
1997
Altered calcium homeostasis in the rat cochlear duct and endogenous corticosteroid insufficiency.
European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology – Head and Neck Surgery.
254(4):165-8
[PMID] 9151013.
1997
Ca(2+)-ATPases in the cochlear duct.
Acta oto-laryngologica.
117(4):553-8
[PMID] 9288211.
1997
High-level expression of ovine growth hormone in Escherichia coli: single-step purification and characterization.
Protein expression and purification.
11(2):201-8
[PMID] 9367817.
1996
Correlative evidence of hypertension and altered cochlear microhomeostasis: electrophysiological changes in the spontaneously hypertensive rat.
Hearing research.
102(1-2):63-9
[PMID] 8951451.
1996
Efficient expression, processing and secretion of a biologically active mammalian protein by Vibrio cholerae.
FEBS letters.
387(2-3):167-70
[PMID] 8674542.
1996
Molecular cloning of Clostridium perfringens epsilon-toxin gene and its high level expression in E. coli.
Biochemical and biophysical research communications.
226(3):735-40
[PMID] 8831683.
1996
Production of the beta-subunit of human chorionic gonadotropin in Escherichia coli and its export mediated by the heat-labile enterotoxin chain-B signal sequence.
Gene.
173(2):271-4
[PMID] 8964513.
1996
Somatic embryogenesis and regeneration of triploid plants in endosperm cultures of Acacia nilotica.
Plant cell reports.
15(11):855-8
[DOI] 10.1007/BF00233156.
[PMID] 24178224.
1996
Translational fusion of heat labile enterotoxin chain B and beta-subunit of human chorionic gonadotropin: periplasmic expression in Escherichia coli and its immunogenicity.
FEBS letters.
387(1):23-6
[PMID] 8654560.
1995
Autocrine inhibition of Na+/K(+)-ATPase by nitric oxide in mouse proximal tubule epithelial cells.
The Journal of clinical investigation.
95(5):2083-8
[PMID] 7537754.
1995
High cell density fermentation of recombinant Vibrio cholerae for the production of B subunit of Escherichia coli enterotoxin.
Biotechnology and bioengineering.
45(3):245-50
[PMID] 18623144.
1995
High-level production and one-step purification of biologically active human growth hormone in Escherichia coli.
Gene.
165(2):303-6
[PMID] 8522194.
1995
Induction of differentiation in murine erythroleukemia cells by 1 alpha,25-dihydroxy vitamin D3.
Cancer letters.
90(2):225-30
[PMID] 7736459.
1994
In vitro and in vivo effects of ammonium chloride on H-K-ATPase activity in the outer medullary collecting duct.
Contributions to nephrology.
110:67-74
[PMID] 7956259.
1994
Nitric oxide inhibits bafilomycin-sensitive H(+)-ATPase activity in rat cortical collecting duct.
The American journal of physiology.
267(4 Pt 2):F509-15
[PMID] 7524355.
1993
Alpha-2 adrenergic receptors in inner medullary collecting duct cells of the rabbit kidney.
The Journal of pharmacology and experimental therapeutics.
264(2):879-88
[PMID] 8382282.
1993
Alpha-2 adrenoceptors in medullary thick ascending limbs of the rabbit kidney.
The Journal of pharmacology and experimental therapeutics.
266(1):279-87
[PMID] 8392554.
1993
Cholinergic inhibition of Na-K-ATPase via activation of protein kinase C in Madin-Darby canine kidney cells.
Journal of the American Society of Nephrology : JASN.
4(2):195-205
[PMID] 8400083.
1993
Efficient processing and export of human growth hormone by heat labile enterotoxin chain B signal sequence.
FEBS letters.
330(1):61-5
[PMID] 8370461.
1992
Actions of adrenergic and cholinergic drugs on renal tubular cells.
Pharmacological reviews.
44(1):81-102
[PMID] 1557426.
1992
Characterization of cholinergic receptors in Madin-Darby canine kidney cells.
Journal of the American Society of Nephrology : JASN.
3(2):170-81
[PMID] 1327259.
1992
Muscarinic receptors in MDCK cells are coupled to multiple messenger systems.
The American journal of physiology.
263(6 Pt 1):C1289-94
[PMID] 1335690.
1992
Synthesis and release of acetylcholine in the rabbit kidney cortex.
Life sciences.
51(22):1699-703
[PMID] 1435079.
1991
Alpha-1 adrenergic receptors in renal medullary collecting duct cells.
The Journal of pharmacology and experimental therapeutics.
259(3):1081-7
[PMID] 1684813.
1991
Alpha-1-adrenoceptor-mediated phosphoinositide hydrolysis in renal epithelial cells.
Contributions to nephrology.
95:197-206
[PMID] 1666989.
1991
Changes in H-ATPase activity in the distal nephron segments of the rat during metabolic acidosis and alkalosis.
Contributions to nephrology.
92:39-45
[PMID] 1836756.
1991
Multiple functional enhancer motifs of rat ribosomal gene.
Molecular and cellular biochemistry.
104(1-2):155-62
[PMID] 1921995.
1991
Respective roles of H-ATPase and H-K-ATPase in ion transport in the kidney.
Journal of the American Society of Nephrology : JASN.
2(5):949-60
[PMID] 1836966.
1990
Adenosine and its analogs stimulate phosphoinositide hydrolysis in the kidney.
Pharmacology.
40(2):90-5
[PMID] 2161113.
1990
Decrease in N-ethylmaleimide-sensitive ATPase activity in collecting duct by metabolic alkalosis.
Canadian journal of physiology and pharmacology.
68(8):1119-23
[PMID] 2143964.
1990
Effects of low-potassium diet on N-ethylmaleimide-sensitive ATPase in the distal nephron segments.
Renal physiology and biochemistry.
13(3):129-36
[PMID] 1690906.
1990
Stimulation of phosphoinositide hydrolysis by oxytocin in renal epithelial cells.
The Journal of pharmacology and experimental therapeutics.
252(2):552-7
[PMID] 2156051.
1990
Stimulation of phosphoinositide hydrolysis in renal medulla by vasopressin.
Endocrinology.
127(3):1022-7
[PMID] 2167202.
1989
A 37-base pair element in the far upstream spacer region can enhance transcription of rat rDNA in vitro and can bind to the core promoter-binding factor(s).
The Journal of biological chemistry.
264(1):220-4
[PMID] 2642473.
1989
A cis-acting sequence within the rat ribosomal DNA enhancer region can modulate RNA polymerase II-directed transcription of the metallothionein I gene in vitro.
DNA (Mary Ann Liebert, Inc.).
8(5):311-20
[PMID] 2766929.
1989
Cholinergic receptors in renal medullary collecting duct cells.
The Journal of pharmacology and experimental therapeutics.
248(1):12-6
[PMID] 2536424.
1989
Cholinergic stimulation of phosphoinositide hydrolysis in renal medullary collecting duct cells.
The Journal of pharmacology and experimental therapeutics.
248(2):682-6
[PMID] 2537409.
1989
Interaction of a positive regulatory factor(s) with a 106-base pair upstream region controls transcription of metallothionein-I gene in the liver.
The Journal of biological chemistry.
264(4):2134-8
[PMID] 2914896.
1989
Suppression of ouabain-insensitive K-ATPase activity in rabbit nephron segments during chronic hyperkalemia.
Renal physiology and biochemistry.
12(5-6):295-301
[PMID] 2533699.
1988
Cholinergic stimulation of phosphoinositide hydrolysis in rabbit kidney.
The Journal of pharmacology and experimental therapeutics.
244(2):586-91
[PMID] 2831346.
1988
Effects of aldosterone on NEM-sensitive ATPase in rabbit nephron segments.
Kidney international.
34(1):13-7
[PMID] 2902242.
1988
Effects of osmolality on phosphoinositide hydrolysis in renal medulla.
The Journal of pharmacology and experimental therapeutics.
247(2):495-501
[PMID] 2846822.
1988
Ouabain-insensitive K-adenosine triphosphatase in distal nephron segments of the rabbit.
The Journal of clinical investigation.
81(4):1204-8
[PMID] 2965163.
1988
Vasopressin stimulates phosphoinositide hydrolysis in LLC-PK1 cells.
The American journal of physiology.
255(4 Pt 1):C502-7
[PMID] 2845798.
1987
An enhancer element in the far upstream spacer region of rat ribosomal RNA gene.
The Journal of biological chemistry.
262(24):11616-22
[PMID] 3624227.
1987
Effects of hydrochlorothiazide on Na-K-ATPase activity along the rat nephron.
Kidney international.
31(4):918-22
[PMID] 3035268.
1987
Effects of potassium bicarbonate on distal nephron Na-K-ATPase in adrenalectomized rabbits.
Pflugers Archiv : European journal of physiology.
409(1-2):126-31
[PMID] 3039448.
1986
Activities of cathepsins B and L in isolated nephron segments from proteinuric and nonproteinuric rats.
The American journal of physiology.
250(6 Pt 2):F1055-62
[PMID] 3717347.
1986
Differences in renal tubular Na-K-adenosine triphosphatase in spontaneously hypertensive and normotensive rats.
Journal of cardiovascular pharmacology.
8(1):186-9
[PMID] 2419683.
1985
Effects of thyroid hormone on Na-K-adenosine triphosphatase activity along the rat nephron.
The Journal of laboratory and clinical medicine.
106(5):568-72
[PMID] 2997354.
1985
Glucocorticoid effects on Na-K-ATPase in rabbit nephron segments.
The American journal of physiology.
248(4 Pt 2):F487-91
[PMID] 2984945.
1985
Na-K-ATPase in nephron segments of rats developing spontaneous hypertension.
The American journal of physiology.
249(6 Pt 2):F863-9
[PMID] 3000191.
1985
Renal adaptation to potassium in the adrenalectomized rabbit. Role of distal tubular sodium-potassium adenosine triphosphatase.
The Journal of clinical investigation.
76(3):1065-70
[PMID] 2995442.
1985
Sodium-potassium-adenosine triphosphatase in nephron segments of spontaneously hypertensive rats.
The Journal of laboratory and clinical medicine.
106(1):43-6
[PMID] 2989396.
1985
Stimulation of an N-ethylmaleimide-sensitive ATPase in the collecting duct segments of the rat nephron by metabolic acidosis.
Canadian journal of physiology and pharmacology.
63(10):1291-6
[PMID] 2934119.
1984
Acid phosphatase activity in the mammalian nephron.
The American journal of physiology.
247(2 Pt 2):F252-9
[PMID] 6465319.
1983
Glyoxalase I in regenerating mouse liver exposed to carcinogens.
Biochemistry international.
7(2):207-13
[PMID] 6089810.
1983
Surface membrane-associated phosphatidylethanolamine N-methyltransferase activity in L-929 cells.
Archives of biochemistry and biophysics.
220(1):22-30
[PMID] 6830234.
1982
Effect of low potassium-diet on Na-K-ATPase in rat nephron segments.
Pflugers Archiv : European journal of physiology.
394(2):113-7
[PMID] 6289258.
1981
Mineralocorticoid effects on Na-K-ATPase in individual nephron segments.
The American journal of physiology.
240(6):F536-44
[PMID] 6264796.
1981
The effects of chronic ingestion of tritiated water on hexokinase and lactate dehydrogenase enzymes in liver and brain during postnatal development of mice.
International journal of radiation biology and related studies in physics, chemistry, and medicine.
40(6):665-9
[PMID] 6978303.
1978
The effect of acid–base changes on skeletal muscle twitch tension.
Canadian journal of physiology and pharmacology.
56(4):543-9
[PMID] 28823.
1977
Inhibition of carbonic anhydrases of rat liver by halides.
The Journal of pharmacology and experimental therapeutics.
200(2):434-8
[PMID] 839447.
1976
The effect of acetazolamide on the movement of sodium into the posterior chamber of the dog eye.
The Journal of pharmacology and experimental therapeutics.
199(3):510-7
[PMID] 994015.
1976
The effect of acetazolamide on the movements of anions into the posterior chamber of the dog eye.
The Journal of pharmacology and experimental therapeutics.
196(2):510-6
[PMID] 1255495.
1975
Induction of hepatic carbonic anhydrase by estrogen.
The Journal of pharmacology and experimental therapeutics.
192(2):297-302
[PMID] 804034.
1975
The absence of renal bicarbonate reabsorption maxima during carbonic anhydrase inhibition.
The Journal of pharmacology and experimental therapeutics.
194(1):96-102
[PMID] 239223.
1975
The effect of acetazolamide and carbonic anhydrase inhibition on erythrocyte 2,3-diphosphoglycerate content and metabolism.
The Journal of pharmacology and experimental therapeutics.
193(2):639-46
[PMID] 1142109.
1974
Catalytic activity and inhibition of carbonic anhydrase of rat tissues.
Biochemical pharmacology.
23(22):3153-61
[PMID] 4216355.
1974
The effect of sex hormones on rat liver carbonic anhydrase.
The Journal of pharmacology and experimental therapeutics.
189(2):557-62
[PMID] 4208307.
1970
The effect of ouabain and acetazolamide on transport of sodium and chloride from plasma to aqueous humor.
The Journal of pharmacology and experimental therapeutics.
175(2):237-47
[PMID] 5481695.
Contact Details
Phones:
- Business:
- (352) 378-4623
Emails:
- Business:
- lcgarg@ufl.edu
Addresses:
- Business Mailing:
-
PO Box 100267
GAINESVILLE FL 32610 - Business Street:
-
3939 NW 36TH PL
GAINESVILLE FL 32606